Study on UPLC fingerprint and simultaneous determination of 5 components in Frucius Aceris Fabri
Objective:To establish a method of UPLC fingerprint and quantitative analysis of gallic acid,protocat-echuic acid,p-hydroxycinnamic acid,myricitrin and quercitrin for Frucius Aceris Fabri,and to provide a refer-ence for the quality control of Frucius Aceris Fabri.Methods:The determination was performed on a Waters CORTECS UPLC T3 column(150 mm ×2.1 mm,1.6 μm),with mobile phase consisting of acetonitrile-0.1％phosphoric acid by gradually elution at a flow rate of 0.20 mL·min-1.The column temperature was 30 ℃,and detection wavelength was set at 300 nm.The quality of 10 batches of Frucius Aceris Fabri was evaluated by similarity analysis,CA and TOPSIS analysis of fingerprints.Results:The UPLC fingerprint of Frucius Aceris Fab-ri was established,and 14 peaks were selected as the characteristic fingerprint peaks.Five chemical components were identified,which were gallic acid,protocatechuic acid,p-hydroxycinnamic acid,myricitrin and quercitrin.And a quantitative method for the determination of the 5 chemical components was established.Good similarities were found in the established fingerprint through similarity analysis.CA and TOPSIS analysis showed that 10 bat-ches of Frucius Aceris Fabri samples could be clustered into 3 groups.The sample S9 from Jiangxi was classified as class Ⅰ with best quality.The samples from Guangxi and Guangdong were classified as Class Ⅱ with medium quality.The samples S1,S4 and S6 from Jiangxi were classified as Ⅲ with worst quality.The linear relationship of the 5 chemical components was good,and the r values were all above 0.999.The contents of the 5 chemical components in 10 batches of samples were 0.236-0.356 mg·g-1,0.118-0.398 mg·g-1,0.108-0.141 mg·g-1,0.146-0.222 mg·g-1 and 0.046-0.104 mg·g-1,respectively.Conclusion:The established UPLC fingerprint and quantitative analysis methods of Frucius Aceris Fabri are precise and stable,which can be used for evaluating and controlling the quality of Frucius Aceris Fabri.
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