Simultaneous determination of ten constituents in compound Shuanghua tablets by HPLC
Objective:To establish an HPLC method for simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,forsythiaside A,3,4-O-dicaffeoylquinic acid,3,5-O-dicaf-feoylquinic acid,4,5-O-dicaffeoylquinic acid,forsythin,andrographalide and dehydroandrographalide in com-pound Shuanghua tablets.Methods:The samples were extracted with 50％methanol solution under ultrasonic condition,and were performed on Agilent Zoabax SB-C18 column(250 mm ×4.6 mm,5 μm)by gradient elution of acetonitrile-0.15％phosphoric acid solution at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃,the injection volume was 10 μL,and the detection wavelength were set at 327 nm(detecting neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,forsythiaside A,3,4-O-dicaffeoylquinic acid,3,5-O-dicaf-feoylquinic acid and 4,5-O-dicaffeoylquinic acid)and 226 nm(detecting forsythin,andrographalide and dehydroandrographalide).Results:The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,forsythiaside A,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid,forsythin,andrographalide and dehydroandrographalide were 4.071-40.71 μg·mL-1(r=0.999 9),20.16-201.6 μg·mL-1(r=0.999 9),4.730-47.30 μg·mL-1(r=0.999 9),4.536-45.36 μg·mL-1(r=0.999 8),1.817-18.17 μg·mL-1(r=0.999 9),2.266-22.66 μg·mL-1(r=0.999 7),3.321-33.21μg·mL-1(r=0.999 9),3.462-34.62 μg·mL-1(r=0.999 6),2.111-21.11 μg·mL-1(r=0.999 9)and 2.290-22.90 μg·mL-1(r=0.999 7),respectively.The average recoveries(n=6)were 101.3％(2.1％),103.0％(1.5％),100.9％(2.0％),101.1％(2.0％),98.4％(1.6％),102.2％(2.4％),98.2％(1.3％),97.8％(2.0％),99.0％(2.0％)and 96.4％(1.1％),respectively.The contents of the 10 components in 4 bacthes of Fufang Shuanghua tablets were in the range of 1.685-2.649 mg·g-1,12.202-13.780 mg·g-1,2.415-2.594 mg·g-1,1.340-1.919 mg·g-1,0.501-0.791 mg·g-1,0.891-1.342 mg·g-1,1.299-2.105 mg·g-1,1.147-1.504 mg·g-1,0.654-0.694 mg·g-1,0.846-1.151 mg·g-1,respectively.Conclusion:This simple accurate reproducible method can be used for the quality control and evaluate of compound Shuanghua tablets.
compound Shuanghua tabletsHPLCchemical constituentscontent determinationquality control
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