Determination the content of sodium caprylate in human blood albumin products by ion chromatography
Objective:To establish an ion chromatography method for determination the content of sodium capry-late in human blood albumin products.Methods:The samples were precipitated with eluent,the suspension was centrifuged and filtered,the filtrate was injected to IC,and heptanoic acid was used as the internal standard.A Dionex InPacTM NS1 Analytical Column(250 mm ×4mm,10 μm)and a Dionex InPacTM NG1 Guard Column(35 mm ×4 mm,10 μm)were used,the flow rate was 1.0 mL·min-1.The conductivity detector and ASRS 300 membrane suppressor were used,and the regenerant solution was 5 mmol·L-1 tetrabutylsodium hydroxide solution;the column temperature was 30 ℃ and the injection volume was 25 μL.Results:The resolution between the peaks of sodium caprylate and the internal standard was greater than 1.5,and the linearity of concentration of sodium caprylate was good in the range of 0.38-2.52 mmol·L-1,r=0.999 5(n=6).The RSD of the repeat-ability test was 1.1％(n=6).The average recovery was 97.4％and RSD was 1.8％(n=9).The limits of quantification and detection were 0.19 nmol and 0.09 nmol,respectively.The determination results of the content of sodium octanoate in 20 batches of human blood albumin samples from 7 enterprises at home and abroad ranged from 0.073-0.163 mmol·g-1.Conclusion:The method established in this study is simple to operate,accurate in results,high in sensitivity and good in repeatability,can be used for the determination of sodium caprylate con-tent in human blood albumin products and provide a method guarantee for its quality control.
ion chromatographyinternal standard methodsodium caprylatehuman blood albuminquality control
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