Consistency study on in vitro bioactivity of lactobacillin granules
Objective:To compare the in vitro bioactivity between a generic lactobacillin granules drug and three commercial lactobacillin granules drugs,this study investigated inhibition on pathogenic bacteria,the growth pro-moting effect on probiotics,and establish a method to evaluate the in vitro bioactivity consistency of drugs that reg-ulate gut microbiota.Methods:Two culture systems were set up to investigate the inhibitory effect on pathogenic bacteria and the growth promoting effect on probiotics by lactobacillin granules.The in vitro bioactivity consistency of four products was evaluated by microbial growth curve and analyzed by two-way analysis of variance with Dun-nett-t test.Results:No significant difference(P＞0.05)was observed on inhibition of Staphylococcus aureus and growth promotion of Lactobacillus rhamnosus between the generic lactobacillin granules and the commercial lactobacillin granules.Conclusion:This method could be used to evaluate the in vitro bioactivity of drugs that regulate gut microbiota,and provided guidance on relevant drug development and quality evaluation.
lactobacillin granulein vitro bioactivitygrowth promoting test in vitroin vitro bacteriostatic testcon-sistency evaluation
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