Rapid determination of 7 mycotoxin contaminants in Jianqu by UPLC-MS/MS
Objective:To establish a method for determination of 7 mycotoxin contaminants in Jianqu by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:The samples were extracted with acetonitrile aqueous solution(containing 0.1％formic acid),and purified by QuEChERS ex-traction salt bag and Oasis PRiME HLB solid phase extraction column.The column of UPLC was WATERS HSS T3(100 mm ×2.1 mm,1.8 μm).Mobile phase was acetonitrile-5 mmol·L-1 ammonium formate solution(con-taining 0.1％formic acid)with gradient elution.Ionization mode was electrospray ionization(ESI)with positive mode.The work mode was multiple-reaction monitoring mode(MRM).Results:The developed method provided a good linearity for the 7 mycotoxins with their respective linear rangers.The correlation(r)ranged from 0.999 1 to 0.999 9.The average recoveries ranged from 83.5％to 113.8％,RSDs of 2.1％to 5.2％.Among 10 samples were selected for analysis,aflatoxin B1 was detected from 3 samples at concentration of 1.32 μg·kg-1 to 6.15μg·kg-1.The results indicated that there were serious safety risks in the Jianqu products.Conclusion:This paper establishes a method for the determination of 7 mycotoxins in Jianqu.The residual of mycotoxins in Jianqu can be rapid ly detected by UPLC-MS/MS which is suitable for the risk monitoring of mycotoxins in Jianqu.This article reveals the current situation of mycotoxins contamination in Jianqu,providing a research basis for the safety control of traditional Chinese medicine.It is recommended that the relevant departments should strengthen the corresponding supervision work.
UPLC-MS/MSrapid determinationJianqumycotoxinsquality controltraditional Chinese medicine
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