Application of ginsenoside fingerprint in the quality control of Panax quinquefolius L.from Shandong
Objective:To establish the fingerprint of Panax quinquefolius L.from Shandong by UPLC-PDA,and to simultaneously determine the contents of 16 ginsenosides.Methods:The chromatographic column was an Acquity UPLC BEH C18 column(50 mm ×2.1 mm,1.7 µm),which was eluted with water-acetonitrile by gradi-ent at a flow rate of 0.4 mL·min-1.The detection wavelength was 203 nm,the column temperature was 30 ℃,and the injection volume was 2 μL.The Chinese Pharmacopoeia"Chinese Medicine Chromatography Fingerprint Similarity Evaluation System(2012 Edition)"was used for evaluation,and 42 batches of Panax quinquefolius L.from different habitats were compared with cluster analysis and principal component analysis.Results:The total content 16 ginsenosides in Panax quinquefolius L.from in Shandong were 19.73-58.07 mg·g-1,and the average value was(34.72±8.22)mg·g-1.The fingerprints of the ginsenosides in Panax quinquefolius L.from Shandong were established,and the similarities were above 0.90.And 10 common peaks constituted the charac-teristic peaks of Panax quinquefolius L..Cluster analysis and principal component analysis showed that the con-tents of ginsenosides in Panax quinquefolius L.from Shandong were stable.Conclusion:The fingerprint of Panax quinquefolius L.established is highly characteristic,and the method issimple,which provides data support for the identification and quality control of Panax quinquefolius L..
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