人参皂苷指纹图谱在山东产西洋参质量控制中的应用
Application of ginsenoside fingerprint in the quality control of Panax quinquefolius L.from Shandong
张燕停 1陆雨顺 1张悦 1任利鹏 2魏晓明 2孙印石1
作者信息
- 1. 中国农业科学院特产研究所,长春 130112
- 2. 山东省威海市文登区农业农村局,威海 264400
- 折叠
摘要
目的:利用超高效液相色谱-二极管阵列检测器(UPLC-PDA)法建立山东产西洋参皂苷类成分的指纹图谱,并同时测定16个单体皂苷的含量.方法:采用Acquity UPLC BEH C18(50 mm ×2.1 mm,1.7μm)色谱柱,以水-乙腈为流动相进行梯度洗脱,流速0.4 mL·min-1,检测波长203 nm,柱温30 ℃,进样量2 μL.运用《中国药典》"中药色谱指纹图谱相似度评价系统(2012版)"进行评价,结合聚类分析和主成分分析对42批不同产地的西洋参进行比较.结果:山东产西洋参中16个单体皂苷总量为19.73～58.07 mg·g-1,均值为(34.72±8.22)mg·g-1.建立了西洋参皂苷类成分的指纹图谱,相似度均达到了 0.90以上,确定了 10个共有成分峰构成西洋参的特征峰,聚类分析和主成分分析表明该地区西洋参皂苷类成分具有较好的稳定性.结论:建立的西洋参药材指纹图谱特征性强,方法简便,为山东产西洋参的鉴定和质量控制提供数据支持.
Abstract
Objective:To establish the fingerprint of Panax quinquefolius L.from Shandong by UPLC-PDA,and to simultaneously determine the contents of 16 ginsenosides.Methods:The chromatographic column was an Acquity UPLC BEH C18 column(50 mm ×2.1 mm,1.7 µm),which was eluted with water-acetonitrile by gradi-ent at a flow rate of 0.4 mL·min-1.The detection wavelength was 203 nm,the column temperature was 30 ℃,and the injection volume was 2 μL.The Chinese Pharmacopoeia"Chinese Medicine Chromatography Fingerprint Similarity Evaluation System(2012 Edition)"was used for evaluation,and 42 batches of Panax quinquefolius L.from different habitats were compared with cluster analysis and principal component analysis.Results:The total content 16 ginsenosides in Panax quinquefolius L.from in Shandong were 19.73-58.07 mg·g-1,and the average value was(34.72±8.22)mg·g-1.The fingerprints of the ginsenosides in Panax quinquefolius L.from Shandong were established,and the similarities were above 0.90.And 10 common peaks constituted the charac-teristic peaks of Panax quinquefolius L..Cluster analysis and principal component analysis showed that the con-tents of ginsenosides in Panax quinquefolius L.from Shandong were stable.Conclusion:The fingerprint of Panax quinquefolius L.established is highly characteristic,and the method issimple,which provides data support for the identification and quality control of Panax quinquefolius L..
关键词
山东产西洋参/人参皂苷/超高效液相色谱/指纹图谱/质量评价/聚类分析/主成分分析Key words
Panax quinquefolius L.from Shandong/ginsenoside/UPLC/fingerprint/quality evaluation/cluster analysis/principal component analysis引用本文复制引用
基金项目
吉林省科技发展计划(20190304015YY)
出版年
2024
NETL
NSTL
万方数据