Quality evaluation of Jinteng Qingbi granules based on UHPLC multi-index component determination and principal component analysis
Objective:To establish a UHPLC method for simultaneously determining sinomenine,neochlorogenic acid,magnolflorine,cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,harpagoside,ligustilide and ammonium glycyrrhizate in Jinteng Qingbi granules,and evalu-ate the quality of Jinteng Qingbi granules combined with principal component analysis.Methods:UHPLC wave-length switching method was employed in the study.The chromatographic separation was performed on a Waters XSelect ® CSH C18 column(150 mm ×4.6 mm,2.5 μm)using methanol-acetonitrile(1∶1)as mobile phase A and 0.2％phosphoric acid aqueous solution as mobile phase B in a gradient mode at 25 ℃.The flow rate was 1.0 mL·min-1,and the UV detection wavelength was chosen at 218 nm for sinomenine during 0-17 min,326 nm for neochlorogenic acid,cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C during 17-25 min and 34-98nm,263 nm for magnolflorine,harpagoside,ligustil-ide and ammonium glycyrrhizate during 25-34 min and 98-125nm,respectively.Furthermore,multiple statisti-cal analysis was conducted on the contents of 11 components in 20 batches of Jinteng Qingbi granules using SPSS27.0 software.Resluts:Satisfactory linearities of sinomenine,neochlorogenic acid,magnolflorine,crypto-chlorogenic acid,chlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,harpago-side,ligustilide and ammonium glycyrrhizate were in the ranges of 14.36-143.61 μg·mL-1(r=0.999 9),7.71-77.15 μg·mL-1(r=0.999 8),9.18-91.83 μg·mL-1(r=0.999 7),10.71-107.07 μg·mL-1(r=0.999 8),12.88-128.80 μg·mL-1(r=0.999 8),5.20-51.95 μg·mL-1(r=0.999 7),5.18-51.84 μg·mL-1(r=0.999 8),5.40-53.95 μg·mL-1(r=0.999 8),2.62-26.16 μg·mL-1(r=0.999 9),6.31-63.06 μg·mL-1(r=0.999 9)and 11.13-111.26 μg·mL-1(r=0.997 6),respec-tively.The average recoveries(n=6)were 98.3％,98.3％,98.5％,98.9％,99.2％,101.0％,98.1％,97.1％,96.8％,98.0％and 98.7％,respectively,with RSDs less than 3.0％.The contents ranges of sinome-nine and other 10 components of 20 batches of Jinteng Qingbi granules samples were 2.206-2.704,1.071-1.403,2.096-2.487,1.321-1.724,2.241-2.612,0.605-0.749,0.363-0.412,0.835-1.020,0.151-0.191,0.791-1.188 and 1.008-1.363 mg·g-1,respectively.The results of principal component a-nalysis showed that the quality differences between batches of continuously produced Jinteng Qingbi granules were relatively smaller,and the comprehensive quality of samples S1,S5 and S7 was relatively better.Conclusion:The established UHPLC method of multi-index component determination is simple,accurate and stable.Combined with principal component analysis,it can be used for quality evaluation of Jinteng Qingbi granules comprehensively.
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