Quality evaluation of Japanese encephalitis inactivated vaccine strain in human diploid cells
Objective:To evaluate the quality of the Japanese encephalitis inactivated vaccine strain adapted in human diploid cells(ZFB-3).Methods:The virus strain was identified by identification test to determine whether it was Japanese encephalitis virus strain,the virus titer was tested in mice brain to evaluate the adaptabili-ty and proliferation ability of the virus seed on human diploid cells(ZFB-3),the sterility test,mycoplasma test,and external virus factor test was conducted for any external contamination.Results:The virus strain was proved as Japanese encephalitis virus and had been well adapted to human diploid cells(ZFB-3)with high virus titer.The virus strain was free from contamination by bacteria,fungi,mycoplasma or exogenous viral factors.Conclusion:The quality of Japanese encephalitis inactivated vaccine strain adapted in human diploid cells(ZFB-3)meets the requirements and can be applied to vaccine production.
Japanese encephalitis virusinactivated vaccineshuman diploid cellsvirus strainquality evaluation
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