Simultaneous determination of 11 components in Hongwushen capsules by UPLC-MS/MS
Objective:To establish an UPLC-MS/MS method for simultaneous determination of 11 components(caffeic acid,afzelin,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,salidroside,eleuth-eroside B,ginsenoside Re,ginsenoside Rg1,ginsenoside Rd)in Hongwushen capsules.Methods:A Shim-pack GIST C18 chromatographic column(100 mm ×2.1 mm,2 μm)was used.The mobile phase was composed of ace-tonitrile-0.1％acetic acid-1 mmol·L-1 ammonium acetate aqueous solution and the gradient elution was ap-plied.The flow rate was 0.3 mL·min-1,the column temperature was 30 ℃,and the injection volume was 1μL.Electrospray ionization(ESI)source and multiple reaction monitoring(MRM)mode in both positive and negative were used for ion detections.Results:The linear relationship of 11 components was good in the concen-tration range,and the linear correlation coefficients were all above 0.999 0.The RSD values of precision were less than 3％.The repeatability and stability were good,and the RSD values were less than 5％.The average recover-ies were in the range of 97.1％-101.5％with RSDs≤3.7％.The contents of caffeic acid,afzelin,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,salidroside,eleutheroside B,ginsenoside Re,gin-senoside Rg1 and ginsenoside Rd in 10 batches of Hongwushen capsules were 0.010-0.013 mg·g-1,0.000 7-0.001 4 mg·g-1,0.035-0.038 mg·g-1,0.312-0.315 mg·g-1,0.413-0.417 mg·g-1,0.411-0.416 mg·g-1,4.355-4.358 mg·g-1,0.030-0.032 mg·g-1,0.993-0.999 mg·g-1,1.120-1.124 mg·g-1 and 2.536-2.538 mg·g-1,respectively.Conclusions:The method is accurate,sensitive,stable and reproduci-ble,and can be used for the quality control of Hongwushen capsules.
Hongwushen capsuleRhodiola crenulataAcanthopanax senticosusginsengbig health productssalidrosideginsenoside RdUPLC-MS/MSmultiple reaction monitoringmulti-component content determi-nationquality control
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