Objective:To establish a rapid and accurate ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method for the detection of dextromethorphan and one metabolite dextrorphan in hair.Methods:The hair sample containing dextromethorphan and dextrorphan were extracted with methanol containing internal standard proadifen hydrochloride(SKF525A).The extract was filtered with 0.22 μm organic filter membrane and detected by UPLC-MS/MS.All components were separated by an ACQUITY UPLC HSS T3 column(100 mm ×2.1 mm,1.8 μm),using a gradient elution procedure consisting of 0.2％formic acid(10 mmol·L-1 ammonium formate)and acetonitrile,at a flow rate of 0.3 mL·min-1,and the column temperature was room temperature.Positive electrospray ionization was performed using multiple reaction monitoring mode(MRM).Results:The linear relationships of dextromethorphan and dextrorphan were good in the range of 1-100 ng·mL-1.The linear equations were Y=1.349 49X-0.020 80(r=0.998 8)and Y=0.775 10X-0.013 87(r=0.999 1),respectively.The detection limit and quantitation limit were 0.010 ng·mL-1 and 0.025 ng·mL-1 respectively.Their recoveries ranged from 97.0％-104.8％.The intra-day RSD and inter-day RSD were 1.5％-3.9％and 2.1％-5.5％,respectively.The method was applied to cases,and the results showed that dextromethorphan and dextrorphan were detected in the hair of 6 abusers.Conclusion:This method is simple and sensitive enough to be applied to detect dextromethorphan and one metabolite dextrorphan in hair.
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)hairdextromethorphandextrorphanforensic toxicology
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