Study on quality control of Arnebiae Radix based on"color discrimination grading"
Objective:To determine the color of Arnebiae Radix,and the contents of six main purple pigment components(acetylshikonin,β-acetoxyisovalerylalkannin,deoxyshikonin,isobutylshikonin,β,β'-dimethyl-acrylalkannin and isovalerylshikonin)in Arnebiae Radix,and to study on the correlation between the color of Arnebiae Radix and the contents of six main purple pigment components.Methods:The L,a,and b values of the sample powder were determined using a spectrophotometer to characterize the color of Arnebiae Radix.The Inter-national Commission on Illumination(CIE)had developed a Lab color model,which was a digital description of human vision.A higher L value indicated greater brightness,a higher a value indicated redness and a lower a val-ue indicates greenness,and a higher b value indicated yellowing and a lower b value indicates blueness.The con-tents of purple pigment components were determined using high performance liquid chromatography(HPLC),and the correlation between L,a,and b values and the contents of six main purple pigments was calculated using SPSS software.Results:The contents of acetylshikonin in 135 batches of samples ranged from 0.01％to 3.39％.The contents of β-acetoxyisovalerylalkannin ranged from 0.00％to 1.95％.The contents of deoxyshikonin ranged from 0.00％to 0.23％.The contents of isobutylshikonin ranged from 0.01％to 1.13％.And the contents of isovalerylshikonin ranged from 0.02％to 2.88％.The contents of β,β'-dimethylacrylalkannin ranged from 0.01％to 2.17％.There was a significant negative correlation between the contents of acetylshikonin,β-acetoxyisovalerylalkannin and isobutylshikonin and the L(black_white)chromaticity value of Arnebiae Radix,with a Spearman correlation coefficients between-0.138 and-0.222.The chromaticity value of a(red_green)was related to the five components other than acetylshikonin,which were β-acetoxyisovalerylalkan-nin,deoxyshikonin,isobutylshikonin,β,β'-dimethylacrylalkannin,and isovalerylshikonin,with a spearman correlation coefficients between 0.176 and 0.355;b(blue_yellow)chromaticity value was related to the five com-ponents other than β-acetoxyisovalerylalkannin,which were acetylshikonin,deoxyshikonin,and isobutylshiko-nin,β,β'-dimethylacrylalkannin.β,β'-dimethylacrylalkannin was positively correlated with a coefficient of 0.290,and negatively correlated with the other four components with a coefficients between-0.325 and-0.633.Conclusion:It is recommended that the assay limits of Arnebiae Radix be revised to β,β'-dim-ethylacrylalkannin not less than 0.30％and isovalerylshikonin not less than 0.29％.
Arnebiae Radixcolor and lustreacetylshikoninβ-acetoxyisovalerylalkannindeoxyshikoninisovalerylshikoninβ,β'-dimethylacrylalkanninisovalerylshikonincorrelation analysis
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