Simultaneous determination of seven flavanoids in Artemisiae Argyi Folium by quantitative analysis of multi-components by single marker
Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)for simultaneous determination of 7 flavanoids(5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidu-lin,kaempferol,jaceosidin,eupatilin and casticin)in Artemisiae Argyi Folium.Methods:The HPLC system consisted of the Agilent ZORBAX SB-C18 column(150 mm ×4.6 mm,5 μm)column with gradient elution of acetonitrile and 0.2％phosphoric acid as the mobile phase at a flow rate of 1.0 mL·min-1,a detection wave-length of 350 nm,and a column temperature of 30 ℃.Eupatilin was selected as the internal reference substance,the relative correction factors between eupatilin and the other 6 flavanoids were established,and the contents of these 7 constituents in samples were calculated to realize QAMS.At the same time,compared with the external standard method to verify the accuracy and feasibility of the QAMS method.Results:Within a certain linear range,the relative correction factors between eupatilin and 5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidulin,kaempferol,jaceosidin as well as casticin were 0.958,1.387,1.000,0.950,0.957 and 1.297,respec-tively(RSDs of RCFs were less than 2.0％).The contents of 5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidulin,kaempferol,jaceosidin,eupatilin,casticin in 20 batches of Artemisiae Argyi Folium were 0.031 4-0.623 5 mg·g-1,0.000 9-0.092 6 mg·g-1,0.020 6-0.170 7 mg·g-1,0.011 0-0.184 7 mg·g-1,0.011 7-0.864 0 mg·g-1,0.253 2-2.555 0 mg·g-1 and 0.015 6-0.250 7 mg·g-1,respectively.Conclusion:Using eupatilin as the internal reference,QAMS method for 7 flavanoids is established.The method is accurate and reliable,and can be used for quality control and quantitative analysis of Artemisiae Argyi Folium.
NETL
NSTL
万方数据
