Study on HPLC fingerprint and determination of 6 components in Mongolian medicinal material Tournefortia sibirica Linnaeus
Objective:To establish HPLC fingerprint and determine caffeic acid,isoquercitrin,quercitrin,rosmarinic acid,lithospermic acid and salvianolic acid B in Tournefortia sibirica Linnaeus,and to provide evidence for quality control of Tournefortia sibirica Linnaeus.Methods:The chromatographic separation was performed on an Agilent Eclipse Plus C18 column(250 mm ×4.6 mm,5 μm)with gradient elution(0-60 min,9％B→38％B)of 0.2％phosphoric acid(A)and acetonitrile(B).The detection wavelength was 330 nm.The column temperature was kept at 40 ℃ and the flow rate was 1.0 mL·min-1.The HPLC fingerprint of all batches of Tournefortia sibirica Linnaeus was established using Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition)and the common peaks were identified by reference standards.Six constituents in Tourne-fortia sibirica Linnaeus were quantified.Results:Eleven common peaks were confirmed,and 6 common peaks were identified by reference standards including caffeic acid,isoquercetin,quercetin,rosmarinic acid,purple oxalic acid,and salvianolic acid B.The similarities of 17 batches samples were 0.931 to 0.996.By the methodology exam-ination,RSDs for the precision test were 1.9％,1.0％,1.4％,0.19％,1.1％and 0.32％,respectively.RSDs for the reproducible test were 3.5％,2.3％,3.3％,0.14％,1.1％and 0.19％,respectively.RSDs for the stabil-ity test were 0.80％,1.1％,1.7％,0.52％,0.54％and 0.78％,respectively.Caffeic acid,isoquercitrin,quer-citrin,rosmarinic acid,lithospermic acid and salvianolic acid B had good separation and showed good linearity in their respective linear ranges.The average recoveries ranged from 95％to 105％,and the contents(calculated with reference to the dried drug)were between 0.004％to 0.013％,0.030％to 0.259％,0.032％to 0.256％,0.256％to 1.246％,0.018％to 0.072％and 0.062％0.499％,respectively.Conclusion:The established HPLC fingerprint and quantifi-cation method is stable and reliable,which can provide basis for the quality control of Tournefortia sibirica Linnaeus.
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