Quality differences of Puerariae Flos with different commercial specifications based on HPLC characteristic chromatogram,chemometrics and multi-index component determination
Objective:To establish HPLC characteristic chromatogram and multi-index component determination methods,so as to effectively distinguish Pueraria lobata(Willd.)Ohwi(GH),Pueraria thomsonii Benth(FGH)and their confusing species,and to evaluate the quality of Puerariae Flos.Methods:HPLC was used to analyze the chemical components from different Puerariae Flos with different commercial specifications.The chro-matographic conditions were optimized,and the characteristic chromatogram was established.Principal component analysis(PC A)and partial least squares discriminant analysis(PLS-DA)analysis were used to analyze the char-acteristic chromatogram data of Puerariae Flos with different commercial specifications and to select the differential markers of Puerariae Flos.The differential markers were identified by comparing them with the reference sub-stance,and their contents were determined and the determination results were analyzed.Results:HPLC charac-teristic chromatograms of GH,FGH and their confusing species were established,and a total of 18,27,18 and 8 common peaks were matched in GH,FGH,Pueraria lobata var.montana(GMM)and Wisteria sinensis(Sims)Sweet(ZTH)respectively.Ten chromatographic peaks were identified,including puerarin(Pu),daidzin(Da),daidzein(Dae),genistin(Ge),genistein(Gee),kakkalide(Ka),glycitin(Gl),tectoridin(Td),tectorige-nin(Tg)and tectorigenin-7-O-xylosylglucoside(Tx),and HPLC method for the determination of the above ten components was established simultaneously.The results of PCA and PLS-DA could distinguish four kinds of samples with different commercial specifications.Gl,Tx,Ge,Td,Ka and one unknown compound 6 were the com-ponents with significant differences between the samples with four different commodity specifications.The chemical components of GH and FGH were obviously different from their adulterants.The content of Ka in GH were 10 times as much as that in FGH,and the content of Ge was half as much as that in FGH.Da,Gl was not detected in GH.Conclusion:The established characteristic chromatogram,chemometrics and multi-index component determination method in this study is rapid,simple,objectively and effectively and comprehensive quality evaluation of Puerariae Flos,which can provide the references for improving the quality control standard of Puerariae Flos.
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