Simultaneous determination of linalool and protocatechuate in Homalomena occulta by quantitative nuclear magnetic resonance(qNMR)spectroscopy
Objective:To establish a hydrogen nuclear magnetic quantitative method which can rapidly determine the contents of linalool and protocatechuate in Homalomena occulta.Methods:Using dimethyl sulfoxide-d6(DMSO-d6)as the test solvent and pyrazine-as the internal standard,1H-q NMR measurement was per-formed on a 400 MHz spectrometer.The quantitative resonance peaks of pyrazine,Linalool and protocatechuate were δ8.66,δ5.90 and δ8.60,respectively.Results:The linear correlation coefficients of linalool and proto-catechuate were good with r of 0.999 7 and 0.998 6,respectively.The RSDs of precision were 0.30％and 0.40％,and the recoveries were 98.8％-100.7％(RSD=3.7％)and 99.4％-100.6％(RSD=4.4％),respectively,which indicated that the established method was accurate,stable and feasible.And the whole detec-tion process was completed in about three minutes.The contents of 2 components in 4 batches of Homalomena occulta were 0.328-0.398 mg·g-1(linalool)and 0.559-0.630 mg·g-1(protocatechuate),respectively.Conclusion:The 1H-q NMR method has the advantages of simple operation,fast analysis speed and specificity,and can be used for the simultaneous determination of two active components in Homalomena occulta,providing a scientific basis for the overall quality evaluation and quality control of Homalomena occulta.
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