Determination of 47 pesticide resides in sucrose by UPLC-MS/MS
Objective:To establish a UPLC-MS/MS method for the simultaneous determination of 47 pesticide residues in Sucrose.Methods:The residues in the sample were extracted by acidic acetonitrile,concentrated by blow dry with nitrogen gas and then analyzed by using LC-MS/MS with multiple reaction monitoring(MRM).Blank matrix standard curve with internal standard method was used to determine the residue contents.Results:Each substance to be tested had good linearity relationship within certain concentration(r＞0.995).The limits of quantification(LOQ)were 0.01-2.00 μg·kg-1.The recoveries of 47 pesticide residues at three levels of 10,25 and 50 µg·kg-1 were from 71.0％to 106.0％with the RSD of 0.90％-8.5％in sucrose.2 batches in 15 batches of sucrose samples were detected thiamethoxam and thiamethoxam,while the rest were not detected.Conclusion:The method is simple,rapid and characterized with acceptable sensitivity and accuracy to meet the requirements of the determination of analysis,and this developed procedure is suitable for the determina-tion of pesticide residues in Sucrose,can be used for quality control of sucrose.
sucrosepesticide residuesliquid chromatography-mass spectrometrymulti reaction monitoring mode(MRM)matrix effectinternal standard method
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