Determination and preliminary risk assessment of acrylamide in"8 famous medicines from Zhejiang"by isotope-labeled internal standard-UHPLC-MS/MS in combination with QuEChERS method
Objective:To determine the content of acrylamide in"8 famous medicines from Zhejiang",and to carry out the preliminary risk assessment based on the determination results.Methods:After optimization of QuEChERS extraction,the content of acrylamide was determined by the method of isotope-labeled internal standard-ultra high performance liquid chromatography-tandem mass spectrometry(isotope-labeled IS-UHPLC-MS/MS).The determination was carried out with a Phenomenex Kinetex C18 column(100 mm × 2.1 mm,1.7 μm)as the stationary phase,gradient elution was carried out with methanol-0.1％formic acid aqueous solution as the mobile phase,13C3 acrylamide as the internal standard,and multiple reaction monitoring(MRM)mode was used to detect the residual acrylamide.Results:The content ranges of acrylamide in 247 batches of samples were 0-26 403 μg·kg-1.These contents exceeded the regulations stipulated by some nations or organi-zations,and posed certain safety risks in long-term use.Conclusion:The method established in this study is rapid,simple,sensitive and reproducible,and will lay a scientific basis for the quality control and safety evalua-tion of acrylamide in"8 famous medicines from Zhejiang"and other genuine medicinal materials.
acrylamide8 famous medicines from Zhejiangultra high performance liquid chromatography-tandem mass spectrometrycontent determinationrisk assessmentQuEChERS
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