Research on the quality evaluation system of antibacterial activity of Flos Lonicerae extract based on chromatogram-effect correlation
Objective:To establish a chromatogram-effect correlated quality evaluation system for the antibacte-rial activity of Flos Lonicerae extract by data analysis of components.Methods:The best liquid chromatographic conditions were optimized by investigating the conditions of mobile phase,wavelength and flow rate,and the quan-titative fingerprint of 50 batches of Flos Lonicerae extract was established.The best antibacterial concentration of the extract was obtained by microdilution method,and the antibacterial rates of 50 batches of samples were determined.Similarity analysis,cluster analysis,principal component analysis,grey correlation analysis and mathematical model establishment by support vector machine were used to analyze the quantitative fingerprints and antibacterial rates of 50 batches of samples.Results:Eighteen common peaks were selected from the 50 quantita-tive fingerprints of Flos Lonicerae extract,and the similarities were between 0.608-1.Six chemical components were identified(peak 4:neochlorogenic acid,peak 8:chlorogenic acid,peak 9:cryptochlorogenic acid,peak 16:isochlorogenic acid B,peak 17:isochlorogenic acid A and peak 18:isochlorogenic acid C).The average an-tibacterial rates of 50 batches of extracts were between 3.93％-70.50％.The results of principal component anal-ysis and cluster analysis were highly consistent,and the results of grey correlation analysis showed that all compo-nents were positively correlated with antibacterial effect.The relative deviations between the predicted data of the mathematical model and the experimental data were all below 2％.Conclusion:The HPLC conditions of Flos Lonicerae extract are stable and reliable,and the quality evaluation system of antibacterial activity of Flos Lonicerae extract established can evaluate its quality based on antibacterial rate.
Flos Lonicerae extractchromatogram-effect correlationquantitative fingerprintmathematical mod-elantibacterial actionquality evaluation system
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