Quality evaluation of Hyssopus cuspidatu Boriss.based on characteristic chromatogram and chemical pattern recognition
Objective:To establish an HPLC characteristic chromatogram for Hyssopus cuspidatu Boriss.and con-trol quality and identity authentication for the herb.Methods:The HPLC method was used,and the mobile pha-ses were acetonitrile(A)and 0.1％aqueous phosphoric acid(B)in gradient elution(0-15 min,10％A;15-30 min,10％A→15％A;30-40 min,15％A→18％A;40-60 min,18％A→23％A;60-70 min,23％A→55％A;70-85 min,55％A→60％A;85-85.1 min,60％A→10％A;85.1-90 min,10％A).The column temperature was 30 ℃,the flow rate was 1.0 mL·min-1,the detection wavelength was 330 nm and the injection volume was 20 µL.Fifteen batches of Hyssopus cuspidatu Boriss.and its adulterants,notably Cynan-chum officinale,were analyzed to construct characteristic chromatogram.The authenticity and quality of Hyssopus cuspidatu Boriss.were assessed through principal component analysis(PC A),cluster analysis,and similarity anal-ysis.Results:Analysis of the characteristic chromatogram of ten Hyssopus cuspidatu Boriss.batches revealed fif-teen distinct peaks,with six compounds identified as isodendrobioside,molluscoside,rosmarinic acid,salvianolic acid,geranylgeranyl,and trichothecenes.Cluster analysis segregated the Hyssopus cuspidatu Boriss.samples into three distinct groups,primarily influenced by geographical(latitude and longitude)and environmental(climate)factors.PCA results indicated that four principal components accounted for 92.914％of the variance.The similar-ities for the ten batches of Hyssopus cuspidatu Boriss.ranged from 0.896 to 0.997,with no significant difference observed between the wild and cultivated varieties in terms of overall HPLC profile and relative peak content.The overall profiles of the HPLC characteristic chromatogram and the contents of the main components of the Hyssopus cuspidatu Boriss.and the Nepeta bracteata Benth.were significantly different.Conclusion:The HPLC character-istic chromatogram method developed in this study is reproducible,stable,and practical for the quality control and authentication of Hyssopus cuspidatu Boriss.Moreover,it offers a reliable analytical technique for the quality eval-uation and authentication of this herb,providing substantial data support.
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