Optimization of HPLC with cyclodextrin as the mobile phase additive for the determination of ursolic acid and oleanolic acid in Corni Fructus by molecular design and response surface method
Objective:To establish high-performance liquid chromatography method using cyclodextrin as a mobile phase additive for the determination of ursolic acid and oleanolic acid in Corni Fructus,providing a basis for quality control of Corni Fructus.Methods:Molecular design and response surface method were used to opti-mize the determination method of ursolic acid and oleanolic acid in Corni Fructus.Agilent Eclipse XDB C18column(150 mm ×4.6 mm,5 μm)was adopted as the stationary phase,the mobile phase was 3 mmol·L-1 γ-cyclo-dextrin solution(contained 0.05％phosphoric acid)-acetonitrile(60∶40),the flow rate was 1.0 mL·min-1,the column temperature was 25 ℃ and the detecting wavelength was 210 nm.Results:The resolution of chromatographic peaks of ursolic acid and oleanolic acid was 3.81.Linearities were good in the range of 400-4 000 ng for ursolic acid and in the range of 200-2 000 ng for oleanolic acid.The RSDs for precision,reproducibility,and stability were less than 1.4％,1.8％,and 1.7％,respectively.The recovery rates were 98.5％(RSD=1.7％)and 99.1％(RSD=2.0％).The contents of ursolic acid in three batches of Corni Fructus were 2.66 mg·g-1,2.35 mg·g-1 and 2.91 mg·g-1,and those of oleanolic acid were 0.83 mg·g-1,0.78 mg·g-1 and 1.12 mg·g-1,respectively.Conclusion:This method is simple,accurate,reliable,and environmental friendly,and can be used as a quality control for Corni Fructus.
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