Simultaneous determination of nine effective components in Solidaginis Herba by QAMS
Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)method for the simultaneous determination of nine effective components including phenolic acids and flavonoids(neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutoside,isoquercitrin,kaemperol-3-O-ruti-noside,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid),in Solidaginis Herba.Methods:Ultra-high performance liquid chromatography was developed on an ACQUITY UPLC ® HSS T3(100 mm ×2.1 mm,1.8 μm)column with acetonitrile(A)-0.1％phosphoric acid solution(B)as mobile phase in gradient elution.The flow rate was 0.3 mL·min-1,the detection wavelength was 327 nm,the column temperature was 30 ℃,and the injection volume was 1 μL.Chlorogenic acid and rutoside were chosen as the internal standards for phenolic acids and flavonoids respectively to establish the determine cor-rection factors of neochlorogenic acid,cryptochlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaf-feoylquinic acid,4,5-O-dicaffeoylquinic acid isoquercitrin and kaemperol-3-O-rutinoside,respectively,by multi-point correction method and their contents were calculated.The results were compared with the results of external standard method to verify their accuracy.Results:Nine components showed good linear relationships within their own ranges(r ≥0.999 5),whose average recoveries were 98.2％-101.7％with the RSDs of 1.0％-1.7％.The contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutoside,isoquercitrin,kaemperol-3-O-rutinoside,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid,in nine batches of samples were 0.008％-0.014％,0.055％-0.097％,0.010％-0.019％,0.191％-0.412％,0.018％-0.035％,0.116％-0.50％,0.028％-0.048％,0.111％-0.235％and 0.113％-0.182％.The results obtained by QAMS were consistent with those of external standard method(relative average deviations less than 2.8％).Conclusion:The simple,accurate and reproducible method can be used for the quality control of Solidaginis Herba.
NETL
NSTL
万方数据
