Simultaneous determination of 13 components in Yankening tablets by UPLC
Objective:To establish an UPLC quantitative analysis method for the simultaneous determination of 13 components in Yankening tablets,including phellodendrine,coptisine,baicalin,palmatine,berberine,wogono-side,baicalein,aloe-emodin,rhein,wogonin,emodin,chrysophanol,physcion.Methods:Agilent Eclipse Plus C18(100 mm × 2.1 mm,1.8 μm)column was used with acetonitrile-0.1％phosphoric acid as mobile phase,and gradient elution at a flow rate of 0.3 mL·min-1.The detection wavelengths were 210 nm and 254 nm.The column temperature was 40 ℃.Results:Phellodendrine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,berberine hydrochloride,baicalin,wogonoside,baicalein,aloe-emodin,rhein,wogo-nin,emodin,chrysophanol,physcion showed good linear relationships within their concentration range of 0.97-48.63 μg·mL-1,0.95-47.52 μg·mL-1,0.86-43.15 μg·mL-1,0.86-43.19 μg·mL-1,0.89-44.37 μg·mL-1,1.00-49.84 µg·mL-1,1.02-51.01 μg·mL-1,0.97-48.31 μg·mL-1,0.99-49.50 μg·mL-1,1.04-51.80 µg·mL-1,1.00-50.04 μg·mL-1,1.00-49.80 µg·mL-1,1.01-50.64 μg·mL-1.The average recoveries(n=6)were 95.2％,96.7％,95.9％,98.3％,94.1％,97.6％,99.2％,96.6％,95.5％,97.2％,97.0％,97.8％,98.7％,RSD values were all less than 2.0％.The con-tents of the 13 chemical components in 3 batches of samples were 1.367-1.488 mg·g-1(calculated as phello-dendrine hydrochloride),0.378-0.412 mg·g-1(calculated as coptisine hydrochloride),4.611-5.505 mg·g-1,0.324-0.407 mg·g-1(calculated as palmatine hydrochloride),3.665-3.878 mg·g-1(calculated as berberine hydrochloride),1.107-1.682 mg·g-1,0.392-0.941 mg·g-1,0.076-0.105 mg·g-1,0.097-0.116 mg·g-1,1.059-1.213 mg·g-1,0.149-0.167 mg·g-1,0.213-0.239 mg·g-1,0.047-0.059 mg·g-1.Conclusion:The method is accurate,high analysis efficiency,good repeatability,it can be used to control the quality of Yankening tablets.
YankeningUPLCanti-dysentery and anti-inflammatoryphellodendrinecoptisinebaicalinpalmatineberberinewogonosidebaicaleinaloe-emodinrheinwogoninemodinchrysophanolphysciondeterminationquality control
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