目的:建立一测多评(QAMS)法同时测定花椒和竹叶花椒中芦丁、金丝桃苷、异槲皮苷、槲皮苷的含量,比较黄酮成分含量差异.方法:花椒甲醇提取液分析,采用Agilent Eclipse Plus C18(250 mm ×4.6 mm,5 μm)色谱柱,流动相为乙腈(A)-0.1％甲酸水(B),梯度洗脱,体积流量1.0 mL·min-1,柱温35℃,检测波长360 nm,进样量10 μL.以金丝桃苷为内标,计算其他3个成分的相对校正因子,测定花椒和竹叶花椒中4个黄酮成分的含量.结果:4个成分在各自范围内线性关系良好(r≥0.999 9),在花椒和竹叶花椒中的平均加样回收率(n=6)分别为99.3％～105.1％和91.9％～99.8％.花椒和竹叶花椒中4个黄酮成分含量的相对平均偏差分别在0.05％～3.37％和0.02％～3.28％,QAMS法所得结果与外标法(ESM)接近.结论:该方法准确可靠,花椒中黄酮总量显著高于竹叶花椒,可为全面评价花椒和竹叶花椒的质量研究提供参考.
Simultaneous determination of 4 flavonoids in Zanthoxyli Pericarpium and Pericarpium Zanthoxyli Armati by QAMS
Objective:To establish the quantitative analysis of multi-components by single marker(QAMS)meth-od to simultaneously determine the contents of rutin,hyperoside,isoquercetin and quercetin in Zanthoxyli Pericarpi-um and Pericarpium Zanthoxyli Armati,and compare the differences in flavonoids contents.Methods:Methanol extract of Zanthoxyli Pericarpium was analyzed on an Agilent Eclipse Plus C18(250 mm ×4.6 mm,5 μm)chrom-atographic column.The mobile phase was acetonitrile(A)-0.1％formic acid(B)in a gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was 35 ℃,and the detection wavelength was 360 nm.The in-jection volume was 10 μL.Using hyperoside as the internal standard substance,the relative correction factors for the other 3 components were calculate and the flavonoids contents in Zanthoxyli Pericarpium and Pericarpium Zan-thoxyli Armati were determined.Results:The 4 components had good linear relationship within their respective ranges(r≥0.999 9).The average recovery rates of Zanthoxyli Pericarpium were 99.3％-105.1％(n=6),while thoes of Pericarpium Zanthoxyli Armati were 91.9％-99.8％(n=6).The relative average deviations of content of Zanthoxyli Pericarpium were 0.05％-3.37％,and the relative average deviations of content of Peri-carpium Zanthoxyli Armati were 0.02％-3.28％.The results obtained by QAMS method were close to those ob-tained by the external standard method(ESM).Conclusion:This method of QAMS method is accurate and relia-ble.The total amount of flavonoids in Pericarpium Zanthoxyli Armati is significantly higher than that in Zanthoxyli Pericarpium.Which provides reference for the comprehensive evaluation of the quality research of Zanthoxyli Peri-carpium and Pericarpium Zanthoxyli Armati.
quantitative analysis of multi-components by single markerZanthoxyli PericarpiumPericarpium Zanthoxyli Armatiflavonoidshyperosiderutinisoquercetinquercetincontent difference
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