Objective:To analyze the fractions and relative contents of volatile oils of Magnoliae Flos at different harvesting periods,to elucidate the dynamic pattern of changes in the chemical composition of Magnoliae Flos at five harvesting periods,and to evaluate its antioxidant and antimicrobial activities.Methods:The volatile oils of Magnoliae Flos at five harvesting periods was extracted by water vapour distillation,and the chemical composition was analyzed by gas chromatography-mass spectrometry(GC-MS)technique,and the relative content of each constituent was calculated.The constituents of Magnoliae Flos at the five harvesting periods were analyzed by PLS-DA analysis,which was used in combination with the VIP value to screen out the differential compounds.The antioxidant activity of the volatile oil of Magnoliae Flos was determined by ferric ion reducing antioxidant power(FRAP)method,and its in vitro antimicrobial activity was investigated by 96-well plate method.Results:The total volatile oils content of Magnoliae Flos was the highest in samples at the 4th harvesting period(10 February 2023).Thirty-eight components were identified in the volatile oils of Magnoliae Flos,and 12 differential compounds were screened,including γ-muurolene,elemene,8-cadinene and α-terpineol,etc.The relative contents of γ-muurolene,alloaeromadendrene,borneol,camphor and cis-4-thujanol were the largest in samples at the 4th harvesting period,which was basically in line with the trend of the change of volatile oil content.The volatile oils in samples at five harvesting period showed certain antioxidant and antibacterial activities.And that in samples at the 4th harvesting period showed the strongest antioxidant activity and the inhibition ability against all five species of bacteria.Conclusion:The chemical composition of the volatile oils in Magnoliae Flos was basically the same in in samples at five harvesting periods,but there is a significant difference in the relative content of its volatile components in each harvesting period,and it is presumed that the beginning of February is the optimal harvesting period for Magnoliae Flos.
关键词
辛夷/挥发油/气相色谱-质谱联用(GC-MS)/偏最小二乘法判别分析(PLS-DA)/抗氧化/抗菌
Key words
Magnoliae Flos(Xinyi)/volatiles/gas chromatography-mass spectrometry(GC-MS)/partial least squares discriminant analysis(PLS-DA)/antioxidant/antimicrobial
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万方数据