Comparative study of eight components in branches and leaves of Xanthoceras sorbifolia Bunge at different growth stages by UHPLC-MS/MS method
Objective:To establish an UHPLC-MS/MS method for determining eight primary components(cate-chin,epigallocatechin,rutin,quercitrin,epicatechin,(+)-dihydromyricetin,myricitrin and dihydroqurcetin)in the young branches and leaves of Xanthoceras sorbifolia Bunge,a medicinal plant from Mongolia,and to com-pare their contents in samples at different growth stages.Methods:A Waters CORTECS C18(100 mm×2.1 mm,1.6 μm)chromatographic column was adopted using the mobile phase comprised of water containing 0.1％formic acid(A)and acetonitrile(B)with gradient elution(0-1 min,5％B;1-10 min,5％B→28％B;10-11 min,28％B→95％B;11-14 min,95％B;14-15 min,95％B→5％B)at a flow rate of 0.3 mL·min-1.The tem-perature of the column was set at 40 ℃.Injecting volume was 2 μL.Detection was conducted using electrospray ionization(ESI)in negative ion mode with multiple reaction monitoring(MRM).Results:The linearity of the eight chemical components was found to be excellent in the tested concentration ranges,with correlation coefficients above 0.997 6.Precision,repeatability and stability were satisfactory and the average recoveries were between 97.4％and 106.0％with RSDs≤5.0％.In six batches of leaves,contents of catechin,epigallocatechin,rutin,quercitrin,epicatechin,(+)-dihydromyricetin,myricitrin and dihydroqurcetin were in the ranges of 0.090-0.904 mg·g-1,0.093-2.258 mg·g-1,0.001-0.005 mg·g-1,0.530-6.176 mg·g-1,0.158-1.561 mg·g-1,0.002-0.056 mg·g-1,4.008-10.218 mg·g-1 and 1.049-16.990 mg·g-1,respectively.In six batches of young branches,the contents ranged from 0.384-1.025 mg·g-1,0.911-2.427 mg·g-1,0.008-0.127 mg·g-1,0.870-2.295 mg·g-1,0.659-1.746 mg·g-1,0.125-1.079 mg·g-1,2.296-4.681 mg·g-1 and 1.958-4.946 mg·g-1,respectively.The contents of eight components varied a lot in samples from different parts.The contents of myricitrin,rutin and quercitrin in the leaves exhibited noticeable changes with the growth cycle,suggesting their potential as quality control markers for leaves of Xantho-ceras sorbifolia.Conclusion:The method is accurate,sensitive,stable,repeatable,and suitable for simulta-neous determination of eight components in Xanthoceras sorbifolia Bunge,offering reference for quality control of its leaves and branches.
Xanthoceras sorbifolia Bungeleaves and brancheschemical componentsquantitative analysisUHPLC-MS/MSmultiple reaction monitoring(MRM)
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