Assessment of transfer factor bioactivity under 6-mercaptopurine treatment by cell proliferation assay
Objective:To develop a method to assess bioactivity of trasfer factor(TF)based on their protective effcets on Jurkat E6-1 cells.Methods:Proliferative effects of different concentrations of TF on 6-mercaptopu-rine(6-MP)treated Jurkat E6-1 cells detected by CCK-8 assay and the precision was validated.Results:TF exhibited protective activity to change the inhibitory concentration(IC50)value of 6-MP from(0.46±0.10)μg·mL-1 to(1.11±0.30)μg·mL-1 when treated with 20 μg·mL-1 TF.When cells were treated with 6-MP,there is a good linear relationship between the concentration of TF and cell survival rate,r＞0.95.At each concentration level of the dose-response curve,the RSD was less than 20％.The median effective concentration(EC50)of TF was(28.49±9.60)μg·mL-1,and the confidence limit was less than 20％.Conclusions:TF significantly improved Jurkat E6-1 cell survival rate in dose dependent manner when chal-lenged with 6-MP.It may be suitable for evaluate bioactivity of TF.
transfer factor6-mercaptopurine(6-MP)Jurkat E6-1 cellsbioactivity
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