Study on chemical composition difference between ancient and modern Baihe Dihuang decoction based on multi-component analysis
Objective:To study the difference of chemical composition between ancient and modern clinical pre-scriptions of Baihe Dihuang decoction by multi-component analysis.Methods:The polysaccharide extract of Baihe Dihuang decoction was determined by anthrone-sulfuric acid-ultraviolet spectrophotometer under 580 nm ultraviolet light.Six monosaccharides and oligosaccharides in the ancient and modern Baihe Dihuang decoction were determined by UPLC-CAD.An Agilent InfinityLab Poroshell 120 HILIC-Z(3.0 x 100 mm,2.7 μm)col-umn was used.The mobile phase was consisted of A 0.2％ammonia in acetonitrile and 0.2％ammonia in water with gradient elution.The flow rate was 0.8 mL·min-,the column temperature 35 ℃ and the CAD detector nebulizer temperature was 70 ℃.Data acquisition frequency was 10 Hz and the filter was 1.05 s.The injection volumn was 2 μL.The non-polar components were qualitatively and quantitatively analyzed by high performance liquid-quadrupole tandem time-of-flight mass spectrometry(UPLC-Q TOF MS/MS)and high performance liquid-phase tandem triple quadrupole mass spectrometry(UPLC-QQQ MS),respectively.The differential components of ancient and modern Baihe Dihuang Decoction were analyzed and 9 components were determined.Results:The linear relationship of 6 mono-oligosaccharides/polysaccharides and 9 differential components was good(r＞0.999).The average recoveries ranged from 97.7％to 104.9％with RSDs≤3.1％.Precision,repeat-ability and stability met the requirements.The results showed that the conents of all the analytes in ancient and modern Baihe Dihuang decoction were significantly different(P＜0.05).Conclusion:The methods used in this experiment are accurate,sensitive,stable and reproducible,which can provide reference for the quality control and pharmacodynamic material basic research of Baihe Dihuang Decoction.
Baihe Dihuang decoctionancient and modern differencespolysaccharidesmonosaccharidesUPLCdifferent components
万方数据
维普
