Determination of 12 aconite alkaloids in Fengshi Gutong tablets by HPLC
Objective:To establish a method for determination of aconite alkaloids in Fengshi Gutong tablets.Methods:Analysis was performed on a WatersXSelect ® CSH C18(250 nm×4.6 mm,5 μm)column with mobile phase consisting of 0.1％acetic acid aqueous solution,acetonitrile and methanol with gradient elution at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 245 nm and the column temperature was 30 ℃.Results:Twelve aconite alkaloids could be separated well.When the injection amounts of ranaconitine,benzoylmesaconine,benzoylmesaconine,benzoylmesaconine,acoforestinine and beiwutine,mesaconitine,hypaconitine,indaconitine,aconitine,yunaconitine,bulleyaconitine A were 0.022 8-0.136 6 μg,0.041 5-0.249 0 μg,0.033 5-0.200 8 μg,0.033 9-0.203 1 μg,0.033 1-0.198 6 μg,0.040 3-0.241 6 μg,0.030 2-0.181 4 μg,0.028 6-0.171 6 μg,0.033 6-0.201 6 μg,0.030 3-0.181 9 μg,0.063 4-0.381 5 μg and 0.034 0-0.204 2 μg,respectively,the peak area showed a good linear relationship with the injection amounts.The average recovery rates of 12 aconite alkaloid components ranged from 93.6％to 101.5％,and the RSD ranged from 0.55％to 2.6％.The average contents of ranaconitine,benzoylmesaconine,benzoylmesaconine,benzoylmesaconine,acoforestinine and beiwutine,mesaconitine,hypaconitine,indaconitine and yunaconitine in three batches of Fengshi Gutong tablets(batch No.211227,220823 and 230425)were 0.492 μg,65.78 μg,7.319 μg,10.164 μg,1.068 μg,5.583 μg,2.573 μg,5.865 μg,2.021 μg,2.050 μg,respectively.And aconitine and bulleyaconitine A were not detected.Conclusions:The established method is accurate and reliable,and can be used for the determination of 12 aconite alkaloids compounds in Feng-shi Gutong tablets to achieve comprehensive quality control of preparations.
Fengshi Gutong tabletsaconitinebulleyaconitine AHPLCquality control
万方数据
维普
