Establishment of HPLC fingerprints and determination of six components for Zilian Shengji gel
Objective:To establish the HPLC fingerprints for Zilian Shengji gel and to determine the contents of gallic acid,tableberberine,coptisine,palmatine,berberine and shikonin.Methods:The establishment of finger-prints was performed on a 25 ℃ thermostatic Agilent C18-WR(250 mm × 4.6 mm,5 μm)column with the mobile phase comprising of acetonitrile-0.15％phosphoric acid water at the flowing rate of 0.8mL·min-1 in a gradient elution manner,and the detection wavelength were set at 270 nm,345 nm and 516 nm.Cluster analysis,principal component analysis(PC A)and orthogonal partial least squares discriminant analysis(OPLS-DA)were adopted in chemical pattern recognition.The six components identified were quantitatively determined.Results:The HPLC fingerprint of Zilian Shengji gel was constructed with twenty-six common chromatographic peaks,eleven batches of samples with the similarities of 0.828-0.997,six chromatographic peaks of gallic acid,tableberberine,coptisine,palmatine,berberine and shikonin were identified by reference substance compari-son.Six constituents showed good linear relationships within their own ranges(r≥0.999 5)whose average recover-ies were 98.7％-99.3％,with the RSDs of 1.3％-1.8％.The content ranges of the above mentioned six compo-nents in eleven batches of samples were 827.74-1 513.50 μg·g-1,137.52-296.05 μg·g-1,381.83-884.73μg·g-1,2 023.81-4 051.66 μg·g-1,524.15-986.13 μg·g-1 and 47.65-549.46 μg·g-1.Conclusion:This simple,stable,reliable and reproducible method can be used for the quality control of Zilian Shengji gel.
万方数据
维普
