Simultaneous determination of nine constituents in Tongshu Koushuang tablets by HPLC
Objective:To establish an HPLC method for simultaneous determination of loganic acid,gentiopicro-side,hesperidin,paeonol,aloe-emodin,rhein,emodin,chrysophanol and physcion in Tongshu Koushuang tablets(Rhei Radix et Rhizoma,Aurantii Fructus Immaturus and Gentianae Macrophyllae Radix,etc.).Methods:The samples were extracted by refluxing with methanol and the separation was performed on a Thermo Acclaim-C18(250 mm×4.6 mm,5 μm)column at 30 ℃,with mobile phase consisting of 0.15％phosphoric acid solution and acetonitrile with a gradient elution at a flow rate of 1.0 mL·min-1.The injection volume was 10 μL,and the detection wavelength was set at 236 nm(detecting loganic acid),280 nm(detecting gentiopicro-side,hesperidin and paeonol)and 254 nm(detecting aloe-emodin,rhein,emodin,chrysophanol and phy-scion).Results:Nine constituents(loganic acid,gentiopicroside,hesperidin,paeonol,aloe-emodin,rhein,emodin,chrysophanol and physcion)were in good linearity within their own ranges(r were 0.999 1-0.999 9),and the average recoveries were 99.6％(RSD=1.9％),101.1％(RSD=2.0％),103.0％(RSD=1.5％),101.6％(RSD=0.51％),99.8％(RSD=1.4％),100.9％(RSD=1.4％),100.8％(RSD=1.8％),102.7％(RSD=1.2％)and 102.9％(RSD=0.86％),respectively.The content ranges of nine constituents were 1.871-3.517 mg·g-1,6.274-12.55 mg·g-1,2.140-3.946 mg·g-1,1.393-2.018 mg·g-1,0.394 8-0.807 2 mg·g-1,0.657 5-1.246 mg·g-1,0.484 7-0.915 8 mg·g-1,0.839 8-1.429 mg·g-1 and 0.327 3-0.547 9 mg·g-1,respectively.Conclusion:This simple and accurate method can be used for the quality control and evaluation of Tongshu Koushuang tablets.
Tongshu Koushuang tabletsHPLCchemical constituentsloganic acidgentiopicrosidehesperi-dinpaeonolaloe-emodinrheinemodinchrysophanolphyscioncontent determinationquality control
万方数据
维普
