Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)meth-od to simultaneously determine the contents of scopolamine,anisodamine,scopolin,hyoscyamine and scopoletin in Physochlaina infundibularis Kuang.Methods:Agilent 5 TC-C18(250 mm × 4.6 mm,5 μm)column was used with methanol-0.1％phosphoric acid aqueous solution as mobile phase for gradient elution at a flow rate of 1.0 mL·min-1.The detection wavelengths were 210 nm and 344 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.The relative correction factors for scopolamine,scopolin,hyoscyamine and sco-poletin and anisodamine were calculated by using anisodamine as the internal reference substance.The contents were determined and compared with the results of external standard method(ESM).Results:The five compo-nents had good linear relationship in their respective ranges(r=1.000).The relative correction factors of scopol-amine,anisodamine,scopolin,hyoscyamine and scopoletin were 0.951 8,0.562 6,0.830 4 and 0.433 9,respectively,with good repeatability.The contents of scopolamine,anisodamine,scopolin,hyoscyamine and sco-poletin in P.infundibularis from 10 habitats calculated by QAMS method were not significantly different from those determined by ESM.Conclusion:The method is accurate,stable and reproducible,and can be used for the qual-ity control of P.infundibularis.
关键词
华山参/生物碱/一测多评法(QAMS)/含量测定/相对校正因子(RCF)
Key words
Physochlaina infundibularis Kuang/alkaloid/multi-components by single marker(QAMS)/content determination/relative correction factors(RCF)
维普
万方数据