The Single-cell RNA Sequencing Identified the Role of Fibrosis-related Genes in Diabetes-associated Heart Failure
This study aimed to explore the potential link between diabetes and heart failure,focusing on the role of fibrosis-related genes.Utilizing single-cell and transcriptome sequencing data for heart failure from the GEO database and diabetes-related genes from the CTD database,the authors'conducted GO and KEGG pathway enrichment analyses to identify pathways closely associated with diabetes-related heart failure.Patient data collected from March 2017 to March 2019 at the Jiangsu University Affiliated People's Hospital were analyzed,with key gene expression levels measured through real-time PCR.After standardizing the data,differential expression analysis of the GEO database's mRNA expression data identified 206 differentially expressed genes associated with diabe-tes-related heart failure,including 94 upregulated and 112 downregulated genes.Further differential expression analysis between car-diac tissues of diabetic heart failure patients and non-diabetic heart failure patients identified 265 differentially expressed genes,com-prising 136 upregulated and 129 downregulated genes.The PPI network highlighted strong intrinsic connections among the proteins encoded by these differentially expressed genes.Single-cell sequencing analysis indicated that S100A8 was predominantly enriched in cardiac progenitor cells.Subsequent PCR testing of randomly selected patients from both diabetic and non-diabetic heart failure groups revealed significantly higher relative RNA expression levels of S100A8 in diabetic heart failure patients.The result showed that S100A8 is associated with diabetes-induced heart failure through its impact on cardiac progenitor cells,suggesting its potential as a biomarker for Type 2 diabetes-induced heart failure.
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