Study on the Mechanism of Dexamethasone on Microvessel and Energy Metabolism in Hepatocellular Carcinoma
To investigate the effect of dexamethasone on microcirculation angiogenesis and energy metabolism in hepatocellular car-cinoma,HepG2 cells of liver cancer were labeled with green fluorescent protein GFP.The orthotopic liver model was established by orthotopic transplantation.The tumor load of dexamethasone in nude mice was observed by fluorescence imaging system.The golden hamster cheek pouch hepatoma model was established,and the microcirculation growth and tumor growth of the model mice during the drug administration period were recorded under the microscope.Western blot assay was used to detect the expression of gluconeo-genesis related chain kinase(PEPCK)and G6Pase protein in H22 cells of mice under different drug concentrations.The transplan-ted tumor model of liver cancer in mice was established.The expression of PEPCK and G6Pase was detected by WB method,and the expression of vascular endothelial growth factor(VEGF)was detected by double antibody enzyme-linked immunosorbent assay(ELISA).In the control group of nude mice with orthotopic liver cancer,the body weight increased by 27.3％,and the area of liver cancer increased by 6.7 times.In the dexamethasone group,the weight of mice increased by only 0.3％,and the area of liver cancer increased by only 4.7 times.The golden hamster cheek pouch hepatoma model had more tumor bodies and blood vessels than the normal group.The blood vessels in the dexamethasone treatment group grew slowly,only 4.2％after 7 days,and the tumor volume also decreased significantly.The expression of PEPCK and G6Pase in hepatoma H22 cells treated with different concentrations of dexamethasone gradually increased with the increase of drug concentration.The expression of PEPCK and G6Pase in liver cancer transplanted tumor of dexamethasone mice increased gradually with the increase of drug concentration,and the protein expression was 50％higher than that in normal group.Compared with the control group,the expression of VEGF in the experimental group was sig-nificantly reduced,which was inversely proportional to the drug concentration.Dexamethasone can significantly inhibit the growth of liver cancer.Its regulation mechanism may be:On the one hand,it can inhibit energy metabolism by promoting the gluconeogenesis in hepatoma cells;On the other hand,it can reduce angiogenesis and energy supply by inhibiting the expression of vascular endothelial growth factor;Both of the two pathways can inhibit tumor growth.
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