首页|PTEN协同槲皮素抗胃癌细胞增殖、凋亡和上皮-间质转化进程的机制研究

PTEN协同槲皮素抗胃癌细胞增殖、凋亡和上皮-间质转化进程的机制研究

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探讨槲皮素(quercetin，Que)与人第10号染色体缺失的磷酸酶基因(phosphatase and tensin homolog deleted on chromosome ten，PTEN)PTEN 在胃癌细胞 SGC-7901 的增殖、凋亡和上皮-间质转化(epithelial-mesenchymal trans-formation，EMT)的机制研究。通过单独使用不同浓度槲皮素或PTEN转染处理胃癌SGC-7901细胞，分别采用CCK-8法、流式细胞凋亡法及Western blot法检测两者单独使用对胃癌细胞增殖、凋亡和上皮间质转化相关蛋白表达的影响。再通过两者联合处理胃癌细胞，采用RT-PCR法检测基因表达，Western blot法检测蛋白表达，细胞划痕和Transwell小室法检测细胞迁移和侵袭，免疫荧光法检测EMT靶蛋白的表达。槲皮素单独处理细胞时，随着药物浓度的增加，剂量依赖地减弱SGC-7901细胞活性(P＜0。01)，诱导细胞凋亡，并促进PTEN蛋白表达(P＜0。01)，改变抗凋亡蛋白Bcl-2和促凋亡蛋白Bax的表达(P＜0。01)，反转E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)以及转录因子(Slug)的表达(P＜0。01)。PTEN单独转染细胞增加E-cadherin和Bax的基因和蛋白表达(P＜0。05)，降低Bcl-2、Vimentin以及转录因子Slug的表达(P＜0。01)，抑制细胞迁移;两者联合作用后，PTEN增强槲皮素对SGC-7901细胞抗癌能力，进一步抑制肿瘤迁移。PTEN增强槲皮素对胃癌细胞SGC-7901的抗癌作用，这种作用可能是通过共同抑制细胞核转录因子Slug实现的。
Influence of PTEN Combination with Quercetin in the Proliferation,Apoptosis and Epithelial-Mesenchymal Transformation of Gastric Cancer Cells
To investigate the mechanism of quercetin and the human chromosome 10 deletion phosphatase PTEN in proliferation,apoptosis and epithelial-mesenchymal transformation(EMT)of gastric cancer cells SGC-7901,gastric cancer SGC-7901 cells were treated with quercetin at different concentrations or transfected with PTEN.CCK-8 method,flow cytometry method and Western blot method were used to detect the effects of quercetin or PTEN on the proliferation,apoptosis and epithelial interstitial transformation of gastric cancer cells.Gastric cancer cells SGC-7901 were treated by the combination of quercetin and PTEN.RT-PCR was used to detect gene expression,Western blot was used to detect protein expression,and cell migration and invasion were detected by cell scratch and transwell cell assay.The expression of EMT target protein E-cadherin and Vimentin were detected by immunofluores-cence.Quercetin alone decreased the activity of SGC-7901 cells dose-dependent(P＜0.01),induced apoptosis,promoted the expression of PTEN protein(P＜0.01),changed the expression of anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax(P＜0.01),and reversed the expression of E-cadherin,Vimentin and transcription factor(Slug)(P＜0.01).PTEN transfected cells increased the gene and protein expressions of E-cadherin and Bax(P＜0.05),decreased the expressions of Bcl-2,Vimentin and Slug(P＜0.01),and inhibited cell migration.After combined action,PTEN enhanced the anticancer ability of quercetin against SGC-7901 cells and further inhibited tumor migration.PTEN enhances the anticancer effect of quercetin on gastric cancer cell SGC-7901,which may be achieved by inhibiting the nuclear transcription factor Slug.

SGC-7901 cellsQuercetinPTENCell proliferationApoptosisSlugEMT

甘露、郑炜林、秦飞、李杰、雷辰霞、付文涛、余惟一、林乐颖、祝珊珊

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厦门医学院药学系药物检测中心,福建厦门 361026

华侨大学医学院,福建泉州 362000

厦门医学院生物酶催化与药物合成创新研究中心,福建厦门 361026

胃癌SGC-7901 槲皮素 第10号染色体缺失的磷酸酶基因 细胞增殖 凋亡蛋白 转录因子Slug EMT进程

福建省中青年教师自然科技项目厦门市医疗卫生指导性项目厦门医学院大学生创新创业训练计划资助项目厦门医学院大学生创新创业训练计划资助项目厦门医学院大学生创新创业训练计划资助项目厦门医学院大学生创新创业训练计划资助项目

JAT2204013502Z20244ZD11390S202112631009202312631015202412631013202412631073

2024

10.19526/j.cnki.1005-8915.20240403

药物生物技术
中国药科大学,中国医药科技出版社,中国药学会

药物生物技术

CSTPCD
影响因子：0.463
ISSN：1005-8915
年,卷(期)：2024.31(4)