Evaluation of Antibacterial Activity of Biodegradable Nanosystem Contai-ning Gentamicin Sulfate
The primary aim of this research is to develop a novel biodegradable nanosystem that leverages both photothermal therapy and chemotherapy for combating bacterial infections.This system seeks to provide a synergistic approach to enhance antibacterial effica-cy while ensuring biocompatibility and safety.The study involved a comprehensive characterization of the antibacterial nanosystem using techniques such as malvern particle size analysis,transmission electron microscopy(TEM),UV-visible absorption spectroscopy,and fourier-transform infrared spectroscopy(FTIR).This allowed for an evaluation of the system's size,morphology,dispersion in aqueous solutions,and functional groups.The photothermal performance of the nanosystem was assessed under near-infrared(NIR)light irradia-tion at various concentrations and power levels,including an analysis of its photothermal stability.The drug loading efficiency was deter-mined using the drug's standard curve.Antibacterial efficacy was evaluated through bacterial growth curve assays,providing insights into the antimicrobial activity of the system.Additionally,the biodegradability of the nanosystem was investigated using TEM to observe structural changes over time.The synthesized nanosystem demonstrated uniform particle size distribution and excellent water disper-sibility.Under NIR irradiation,the system exhibited consistent and significant temperature increases,with a noticeable rise of 22.1 ℃ at a concentration of 200 μg/mL.Remarkably,when compared to controls such as polydopamine and gentamicin sulfate,the developed nanosystem,under NIR illumination,showed enhanced antibacterial performance,achieving a bacterial kill rate of up to 90.76％at a lower concentration of 8 μg/mL PDA-GS.This indicates a superior antibacterial capability facilitated by the combined effects of photo-thermal therapy and chemotherapy.The research successfully demonstrates the development of a biodegradable nanosystem that com-bines photothermal and chemotherapeutic strategies for the effective treatment of bacterial infections.The system's excellent antibacterial efficiency,along with its potential for safe bioapplication,highlights the promising avenue of integrating photothermal effects with drug therapy in the realm of antibacterial strategies.This study provides a vital foundation for future efforts aimed at combating bacterial infections through innovative nanotechnology-based approaches.
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