Correlation between the Expression of RNA Binding Proteins MEX3D and TSC22D1 in Cervical Cancer Tissue
To investigate the expression of MEX3D protein and TSC22D1 mRNA in cervical cancer and adjacent tissues,and to ana-lyze the correlation between MEX3D protein and clinical manifestations of cervical cancer patients,a total of 76 cervical cancer tissue samples obtained from patients undergoing surgical resection in Sichuan Provincial Maternal and Child Health Hospital from June 2020 to June 2023,as well as matched adjacent normal tissue samples,were collected.Reverse transcription-fluorescence quantitative PCR(qRT-PCR)was used to detect the expression level of TSC22D1 mRNA in the collected samples,and the positive expression of MEX3D protein in tissue samples was analyzed by immunohistochemistry.Spearman grade analysis was used to compare the correla-tion between the expression of TSC22D1 protein and MEX3D mRNA and clinical features in cervical cancer tissues.The expression level of TSC22D1 mRNA in cervical cancer tissues was significantly lower than that in adjacent normal tissues,and the difference was statistically significant(t=9.508,P＜0.001).Compared with the matched adjacent normal tissues,the positive expression of MEX3D protein in 76 cervical cancer tissue samples was significantly increased(x2=64.083,P＜0.05),and the difference was statistically significant,and whether the expression of MEX3D protein was closely related to clinical stage and lymph node metastasis(x2=11.948,0.169;all P＜0.05),but not with age,depth of invasion,vaginal invasion,parauterine invasion and myometrial inva-sion.Spearman correlation analysis showed that there was a negative correlation between the expression levels of TSC22D1 mRNA and MEX3D protein in cervical cancer tissue samples(r=-0.447,P＜0.05).The RNA-binding protein MEX3D is highly expressed in cervical cancer tissues,which is negatively correlated with the expression of TSC22D1 mRNA,and is involved in the occurrence and development of cervical cancer,which can be used as new therapeutic targets and potential diagnostic methods in the diagnosis and treatment of cervical cancer.
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