Mechanism of Action and Metabolomic Analysis of Atorvastatin in Improving NAFLD
To study the mechanism of atorvastatin in the treatment of nonalcoholic fatty liver disease(NAFLD)and its influence on metabonomics,92 patients with NAFLD who were treated in the authors'hospital from May 2022 to May 2023 were prospectively se-lected for this study and were equally divided into two groups,the control group and the experimental group,each containing 46 pa-tients,according to a lottery.Patients in the control group received conventional clinical treatment,while patients in the experimental group were treated with atorvastatin.Liver function,lipid level and inflammation level indexes were observed before and after treat-ment in the 2 groups,and blood metabolomics testing was performed after treatment using GC/MS technology to find out the differen-tial metabolites and identify the differential metabolic pathways by multidimensional statistics.After treatment,patients in the experi-mental group had lower levels of alanine aminotransferase(ALT),aminotransferase(AST),direct bilirubin(DBiL),and total biliru-bin(TBiL)than those in the control group(P＜0.05);After treatment,patients in the experimental group had lower levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein(LDL-C)than those in the control group,and high-density lipopro-tein(HDL-C)levels were higher than those of the control group(P＜0.05);After treatment,the inflammation levels of both groups were improved,and the levels of interleukin-6(IL-6),tumour necrosis factor-α(TNF-α),and ultrasensitive C-reactive protein(hs-CRP)in the experimental group were reduced more significantly than those of the control group(P＜0.05).The results of GC/MS technique showed that alanine,aminobutyric acid,mannuronic acid,linoleic acid,oleic acid,choline,betaine,glucose,mannose,and aminosugar metabolites were significantly different,and there were four metabolic pathways involved,including amino acid metabo-lism,lipid metabolism,alkali metabolism,and sugar metabolism pathway.The application of atorvastatin has shown remarkable results in the treatment of NAFLD,not only effectively improving the liver function and lipid levels of patients,but also reducing the inflam-matory response.The metabolic pathways involved in the results of the GC/MS technique include amino acid metabolism,lipid metabolism,base metabolism,and sugar metabolism pathways.
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