Effect of Graphene Oxide Supported Arginine Nanosystem on Breast Cancer 4T1 Cells and its Biosafety
To study the effects of graphene oxide supported arginine nanosystem(GO-Arg-SL)on 4T1 cells of breast cancer,and to evaluate its biosafety,the effect of GO-Arg-SL on NO production in 4T1 cells of breast cancer was observed by confocal laser fluores-cence microscopy and flow cytometry.MTT and Calcein AM/PI cell were used to observe the inhibition and killing of4T1 cells,and the toxicity of GO-Arg-SL to 4T1 cells was evaluated.BALB/c mices were used for biosafety experiment,hemolysis was observed,and blood biochemistry and routine were detected.It was shown GO-Arg-SL can produce a large amount of NO in 4T1 cells.GO-Arg-SL can inhibit the growth of 4T1 cells,and the inhibition and killing ability of laser irradiation group are stronger.The hemolysis results were in accordance with the regulations,and the blood biochemistry and routine were maintained within the normal range.So GO-Arg-SL photothermal synergistic NO gas has excellent cell killing ability,and has good biosafety.
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