Comparison of the Methods of Aspiration and Slow-pull in Endobronchial Ultrasound-guided Transbronchial Needle Aspiration for Next Genera-tion Sequencing-compatible Tissue Collection in Patients with Non-small Cell Lung Cancer
To compare the methods of aspiration and slow-pull in endobronchial ultrasound-guided transbronchial needle aspiration(EBUS-TBNA)for next generation sequencing(NGS)-compatible tissue collection in patients with non-small cell lung cancer(NSCLC),a total of 82 patients with NSCLC were enrolled from January 2020 to December 2022.The patients were divided into slow-pull group(n=40)and aspiration group(n=42)according to the collection method.The presence of tissue cores,degree of blood contamination,number of tumor cells and the success rate of NGS were compared between the two groups.There was no signifi-cant difference of age,sex,location of tumor,stage of tumor,and pathological diagnosis between the two groups(P＞0.05).The slow-pull group had higher yield of tissue cores(60.7％vs 47.2％,P=0.034),lower degree of blood contamination(5.0％vs 7.1％,P=0.009),and higher number of tumor cells(median 339 counts vs 106 counts,P＜0.001)than the aspiration method.The success rate of the NGS was also significantly higher in the slow-pull group(95.0％)than those in the aspiration group(69.1％)(P＜0.001).Taken together,the results of the present study indicate that the slow-pull method might be a superior tech-nique for EBUS-TBNA to obtain high-quality tissue samples for NGS in patient with NSCLC.The slow-pull method may therefore contribute to the identification of driver gene mutations and translocations and facilitate personalized treatment of NSCLC.
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