首页|运用GC-MS对三种不同方法提取的艾叶挥发油成分的比较分析

运用GC-MS对三种不同方法提取的艾叶挥发油成分的比较分析

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目的 运用GC-MS对不同提取方法所得的艾叶挥发油成分进行比较分析.方法 分别采用水蒸气蒸馏,正己烷回流,静态顶空提取艾叶挥发油,运用气相色谱-质谱联用技术,结合NIST05数据库对分离的化合物进行鉴定,并应用峰面积归一化法计算各成分的相对含量.结果 3种方法共鉴别出艾叶中80个挥发油成分,其中静态顶空进样59个,水蒸气蒸馏37个,正己烷回流31个.结论 结合水蒸气蒸馏和静态顶空进样两种方法可以建立更全面的艾叶挥发性成分GC-MS表征体系;静态顶空进样在挥发油成分鉴别上存在一定优势.
Analysis of volatile oil from Artemisia argyi Levi.et Vant extracted by three different methods by GC-MS
Objective To analyze the the compositions of volatile oil from Artemisia argyi Levi. et Vant extracted by three different methods by GC-MS. Methods The volatile oil from Artemisia argyi Levi. et Vant were extracted by Steam distillation , Hexane reflux extraction and Static headspace. GC-MS was used for the separation and identification. Results By the three methods, 80 compounds were identified. 59 components were extracted by static headspace, 37 by steam distillation and 31 by hexane reflux extraction. Conclusion Combination of steam distillation and static headspace, sampling was feasible to establish GC-MS representation system for Artemisia argyi Levi. et Vant; there were certain advantages of static headspaee in determination of volatile oil contant.

Artemisia argyi Levi. et VantGC-MSstatic headspacejeucalyptol

李玲、吕磊、董昕、吕狄亚、娄子洋

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第二军医大学药学院分析测试中心,上海200433

第二军医大学东方肝胆外科医院药材科,上海200438

艾叶 气相色谱质谱联用 静态顶空进样 桉油精

2012

药学实践与服务
第二军医大学,中国药学会药事管理专业委员会

药学实践与服务

CSTPCD
影响因子:0.701
ISSN:2097-2024
年,卷(期):2012.30(4)
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