Construction of genetic linkage map of Bupleurum chinense DC. using ISSR and SSR markers
Molecular genetic map is a fundamental organizational tool for genomic research.However,a genetic linkage map for Bupleurum chinense DC.has not been developed.In this study,with the theory of pseudo-testcross.96 F_1 plants from an intraspecifie cross of B.chinense were used as mapping populations.Twenty eight ISSR(inter-simple sequence repeat)primers and 44 SSR(simple sequence repeat)primers were used to detect the polymorphisms between the parental plants，and of them，28 ISSRs and 14 SSRs were selected to analyze the F_1 populations.The map consisted of 13 linkage groups which included 80(72 ISSRs and 8 SSRs)loci，and covered 2 633.9 cM with an average density of33.4 cM.All 13 linkage groups consisted of 2-31 loci ranging in length from 15.4-1 295.7 cM.This map will provide a basis for studies on gene mapping，map-based cloning and maker-assisted selection of important traits in B.chinense.
NETL
NSTL
万方数据
维普
