Study on different factors affecting the bionic enzymatic hydrolysis of icariin
This study aims to observe different factors which affected the bionic enzymatic hydrolysis of icariin into baohuoside Ⅰ and to optimize the reaction conditions in order to provide research foundation for building a novel bionic enzymolysis drug delivery system.To simulate the environment in vivo,37 ℃ was set as the temperature and artificial intestinal juice and gastric juice were selected as the buffer solutions.Taking the conversion of baohuoside Ⅰ as index,the effects of the kinds of enzyme,enzyme activity,substrate concentration,reaction time,pancreatin in artificial intestinal juice and surfactant on the conversion of baohuoside Ⅰ were investigated.The results showed that cellulase,β-glucosidase and snailase were all inactive in artificial gastric juice and no baohuoside Ⅰ generated.Pancreatin in artificial intestinal juice couldn't significantly influence the activity of β-glucosidase or snailase (P ＞ 0.05),but noticeably decrease the activity of cellulase (P ＜ 0.05).In artificial intestinal juice,the conversion of baohuoside Ⅰ was highest by using β-glucosidase,and the optimum reaction conditions were determined as follows:enzyme activity 10 U·mL-1,substrate concentration 1 mg·mL-1,3 g·L-1 rhamnolipid and reaction time 3 h.Under this condition,the conversion of baohuoside Ⅰ was 99.8％.
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万方数据
维普
