采用大孔吸附树脂、正相硅胶、反向半制备等色谱技术,从中药九香虫水提取中分离得到一对Z/E异构化吡啶季胺盐新颖碳骨架类化合物.通过核磁共振、红外、质谱等多种光谱技术鉴定了两个新化合物1和2的结构分别为(Z)-3-(but-1"-en-1"-yl)-1-(2'-hydroxyethyl)-4-propylpyridin-1-ium,命名为 aspongopyridine A 和(E)-3-(but-1"-en-1"-yl)-1-(2'-hydroxyethyl)-4-propylpyridin-1-ium,命名为 aspongopyridine B.此外,还对化合物 1 和 2 的体外抗炎、抗肿瘤、乙酰胆碱酯酶抑制和丁酰胆碱酯酶抑制活性进行了评价,结果表明化合物1和2有微弱的乙酰胆碱酯酶抑制活性.
Abstract
A novel pair of Z/E isomeric compounds with unprecedented carbon skeleton were isolated from an aqueous extract of Aspongopus chinensis Dallas by macroporous resin,silica gel,and semi-preparative high performance liquid chromatography(HPLC).Their structures were identified by nuclear magnetic resonance(NMR),Infrared spectroscopy(IR),Mass spectroscopy(MS)and other spectroscopic methods as(Z)-3-(but-1"-en-1"-yl)-1-(2'-hydroxyethyl)-4-propylpyridin-1-ium,namely aspongopyridine A,and(E)-3-(but-1"-en-1"-yl)-1-(2'-hydroxyethyl)-4-propylpyridin-1-ium,namely aspongopyridine B,respectively.Besides,the anti-inflammatory,anti-tumor,acetylcholinesterase inhibition and butyrylcholinesterase inhibition activities of the compounds 1 and 2 were evaluated.The results showed that compounds 1 and 2 have no anti-inflammatory,anti-tumor,and butyrylcho-linesterase inhibition activities instead of weak acetylcholinesterase inhibition activity.
维普
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