Four pyrazines were isolated from the n-butanol fraction of Hypecoum erectum L.by using various chromatographic methods,including MCI gel,ODS,silica gel and semi-preparative HPLC.The structures of the isolated compounds were identified as hyperectpyrazin A(1),1'S-(6-methylpyrazin-2-yl)-ethane-1',2'-diol(2),2-hydroxymethyl-6-methylpyrazin(3)and pyrazine-2-carboxylic acid(4)by spectroscopy methods(1D NMR,2D NMR,UV,IR,MS,etc.).The absolute configuration of compound 2 was determined by using the Mo2(OAc)4 induced CD analysis for the first time.Compound 1 was a new compound,compounds 2-4 were isolated from H.erectum for the first time.Compounds 1-4 were evaluated for their inhibition against acetylcholinesterase and nitric oxide generation induced by lipopolysaccharide-RAW264.7 macrophage cells.At a concentration of 50 μmol·L-1,compounds 2 and 4 displayed inhibitory effects on acetylcholinesterase with the inhibition rates of 44.40％and 43.99％,respectively.
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