Preparation and in vitro evaluation of polylactic acid nanoparticles containing arginine and glucose oxidase
Hydrogen peroxide(H2O2)and nitric oxide(NO)has a short half-life,low bioavailability,poor tumor targeting and systemic adverse reactions in the physiological environment.In this study,phacoemulsification and nano-precipitation were used to synthesize didecyl dimethyl ammonium bromide(DDAB)/polylactic acid nanoparticles(PLA),then L-arginine(L-Arg)and glucose oxidase(GOx)-loaded nanoparticles(GADP)were prepared,and the in vitro antitumor activity was investigated.The particle size,potential,embedding rate and the ability to produce H2O2/NO of the nanoparticles were investigated.Meanwhile,in vitro cell cytotoxicity against human hepatoma cells(HepG2)was evaluated.The results showed that the prepared L-Arg-DDAB/PLA(ADP)nanoparticles were spherical particles.And the particle size and zeta potential were(225.7±6.33)nm and(+23.5± 0.12)mV,respectively.The adsorption rate of GOx was 87.23％±0.02％.The drug loading of L-Arg was 15.6％± 0.22％.The pH value of glucose solution and the amount of H2O2 showed that GADP had good catalytic activity.In vitro cytotoxicity experiments showed that blank nanoparticles were nontoxic,while the drug-loaded nanoparticles presented enhanced antitumor effect on HepG2 cells.And can inhibit tumor cell migration.The low dose nano-scale NO delivery system GADP can effectively inhibit the migration of tumor cells and kill tumor cells,thus producing therapeutic benefits.
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