摘要
运用硅胶、MCI、Sephadex LH-20等柱色谱,结合半制备液相分离技术对多裂黄檀80％乙醇提取物的乙酸乙酯部位分离纯化得到12个化合物.通过UV、IR、MS、1D/2DNMR等波谱数据与结合文献确定化合物的结构,分别鉴定为多裂黄檀酚A(1)、多裂黄檀酚B(2)、R-(-)-3'-hydroxy-2,4,5-trimethoxydalbergiquinol(3)、neokhriol A(4)、mucronulatol(5)、(3R)-7,2',3'-三羟基-4'-甲氧基异黄烷(6)、异短尖剑豆酚(7)、(3S)-violanone(8)、3'-O-methylviolanone(9)、eryvarin M(10)、(±)-a,3,4,2',4'-pentahydroxydihydrochalcone(11)和(-)-butin(12).其中,化合物1和2为新化合物,化合物3～12为首次从该植物中分离得到.化合物1、2、4、6、8、11、12对DPPH自由基有良好清除作用.
Abstract
Twelve compounds were isolated from the ethyl acetate fraction of the 80％aqueous ethanol extract of the roots and stems of Dalbergia rimosa Roxb.by silica gel,MCI,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Their structures were identified by spectral analysis such as UV,IR,MS,1D/2D NMR and by comparison with literature information as dalbergiquinol A(1),dalbergiquinol B(2),R-(-)-3'-hydroxy-2,4,5-trimethoxydalbergiquinol(3),neokhriol A(4),mucronulatol(5),(3R)-7,2',3'-trihydroxy-4'-methoxy-isoflavane(6),isomucronulatol(7),(3S)-violanone(8),3'-O-methylviolanone(9),eryvarin M(10),(±)-α,3,4,2',4'-pentahydroxydi-hydrochalcone(11)and(-)-butin(12).Compound 1 and 2 are new compounds,and compounds 3-12 were isolated from this plant for the first time.Compounds 1,2,4,6,8,11,12 showed good scavenging effect on DPPH free radical.
基金项目
国家重点研究项目(2019YFC17123012)
广西科技基地和人才专项(桂科AD20238058)
广西中医药局科研项目(GXZYA20220122)
广西一流学科建设开放课题自然科学项目(2019XK129)
广西壮瑶药重点实验室开放基金(GXZY22.2019-10)
维普
万方数据