Three carboline fluorescent probes F1-F3 were designed and synthesized,based on lead compound JYJ-19,an antifungal compound discovered previously by our group.The antifungal activity in vitro results showed that compound F1 had moderate antifungal activity(MIC80=32 μg·mL-1).The stokes shift of Fl is 70 nm.The fluorescent probe F1 has good optical properties and can be used for fluorescence imaging research.Subcellular localization experiments results showed that F1 was enriched in the mitochondria of fungal cells.The detection of intracellular reactive oxygen species levels shows that JYJ-19 enhances intracellular reactive oxygen species levels.The above results indicated that carboline compounds could exert antifungal effects by acting on fungal mitochondria.
关键词
咔啉类化合物/新生隐球菌/荧光探针/亚细胞定位/活性氧
Key words
carboline compound/Cryptococcus neoformans/fluorescence probe/subcellular localization/reactive oxygen species
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