Six compounds were isolated from the roots of Ephedra sinica Stapf using various chromatographic techniques such as silica gel column chromatography,thin layer chromatography and semi-preparative HPLC.Their chemical structures were identified by analysis of physicochemical properties and spectral data,and determined as(Z)-docosanylferulate(1),(E)-docosanylferulate(2),bis(2-ethylheptyl)phythalate(3),2,2'-oxybis(1,4-di-tert-butylbenzene)(4),diisobutyl phthalate(5),bis(2-ethylhexyl)phthalate(6).Among them,compound 1 is a new compound,compounds 2-4 were first isolated from Ephedra.A corticosterone-induced PC-12 cell injury model was used for compound activity screening.The results showed that compounds 1 and 5 significantly improved corticosterone-induced PC-12 cell injury and significantly increased 5-HT7 receptor protein expression in the cells,indicating potential antidepressant activity.
root of Ephedra sinica Stapfchemical constituentsuberin(Z)-docosanylferulateantidepressant activity
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