Study on the chemical constituents of triterpenoids in Patrinia villosa Juss
Seven triterpenoids were isolated and purified from the 95％aqueous EtOH extract whole plants of P.villosa by various chromatographic techniques,such as silica gel,ODS,Sephadex LH-20 gel column chromatog-raphy and preparative high performance liquid chromatography.Based on physicochemical properties and spectral analyses,the structures of the seven compounds were identified as 29-acetoxyoleanolic acid-3-O-a-L-arabinopyran-oside(1),oleanolic acid(2),3β-hydroxy-24-norursa-4(23),12,20(30)-trien-28-oic acid(3),3β-hydroxy-24-nor-urs-4(23),12-dien-28-oic acid(4),ursolic acid(5),hederagenin(6),oleanolic acid 3-O-arabinoside(7).Compound 1 is a new compound.Compounds 3,4,6,and 7 are isolated from P.villosa for the first time.All compounds were assayed for their anti-inflammatory activity by the prodution of NO in lipopolysaccharide-stimulated RAW 264.7 cells.The results showed that compounds 1,2,3,4,6,and 7 significantly inhibited the NO release.
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