摘要
采用硅胶柱色谱、中压液相色谱、葡聚糖凝胶Sephadex LH-20柱色谱及制备液相色谱等多种色谱技术,从白花败酱95％乙醇提取物中分离得到7个三萜类化合物.根据化合物的理化性质和波谱数据确定了它们的结构,分别为29-乙酰氧基齐墩果酸-3-O-a-L-阿拉伯糖苷(1)、齐墩果酸(2)、3β-羟基-24-降-乌苏-4(23),12(13),20(30)-三烯-28-酸(3)、3β-羟基-24-降-乌苏-4(23),12-二烯-28-酸(4)、熊果酸(5)、常春藤皂苷元(6)、齐墩果酸3-O-阿拉伯糖苷(7).化合物1为新化合物,化合物3、4、6、7为首次从该植物中分离得到.通过对脂多糖(LPS)诱导的RAW 264.7细胞(小鼠单核巨噬细胞)释放的一氧化氮(NO)影响实验测定了化合物的抗炎活性,结果表明化合物1、2、3、4、6、7有显著抑制NO作用.
Abstract
Seven triterpenoids were isolated and purified from the 95％aqueous EtOH extract whole plants of P.villosa by various chromatographic techniques,such as silica gel,ODS,Sephadex LH-20 gel column chromatog-raphy and preparative high performance liquid chromatography.Based on physicochemical properties and spectral analyses,the structures of the seven compounds were identified as 29-acetoxyoleanolic acid-3-O-a-L-arabinopyran-oside(1),oleanolic acid(2),3β-hydroxy-24-norursa-4(23),12,20(30)-trien-28-oic acid(3),3β-hydroxy-24-nor-urs-4(23),12-dien-28-oic acid(4),ursolic acid(5),hederagenin(6),oleanolic acid 3-O-arabinoside(7).Compound 1 is a new compound.Compounds 3,4,6,and 7 are isolated from P.villosa for the first time.All compounds were assayed for their anti-inflammatory activity by the prodution of NO in lipopolysaccharide-stimulated RAW 264.7 cells.The results showed that compounds 1,2,3,4,6,and 7 significantly inhibited the NO release.
基金项目
科技重大专项(十三五)(2019ZX09735002-004)
安徽省教育厅高等学校科学研究重大项目(2022AH040323)
安庆医药高等专科学校科创团队项目(KCTD202304)
NETL
NSTL
万方数据