谷胱甘肽促进Emericella sp.1454中emestrin的产量提高
Enhancing production of emestrin in Emericella sp.1454 by adding the biosynthetic precursor glutathione
陈渝川 1肖同美 1苏冰洁 1闫璧滢 1余利岩 1司书毅 1陈明华1
作者信息
- 1. 中国医学科学院、北京协和医学院,医药生物技术研究所,北京 100050
- 折叠
摘要
基于Emericella sp.1454的基因组信息,结合文献分析其次级代谢产物emestrin的生物合成前体,并通过在培养基中添加前体的方法,提高emestrin产量.本研究首次发现在培养基中添加emestrin的生物合成前体—还原型谷胱甘肽可显著提高其产量.每50 g大米培养基中加入1.5 g还原型谷胱甘肽,培养15天,可以得到30.82 mg的emestrin,相较于原始发酵培养方案提升了 11.71倍.该方法既简单经济又高效,为后续高效制备emestrin类化合物奠定了坚实的基础,以及为其他类多硫代二酮哌嗪类化合物的产量提升提供了重要的借鉴.
Abstract
Based on the genomic information of Emericella sp.1454,in conjunction with literature analysis of its secondary metabolite emestrin,this study identified the biosynthetic precursors of emestrin and enhanced its production by supplementing the culture medium with these precursors.In this study,it was found for the first time that the addition of biosynthetic precursor,reduced glutathione,to the culture medium significantly increased emestrin yield.By incorporating 1.5 g of reduced glutathione into 50 g of rice culture medium and fermenting for 15 days,a yield of 30.82 mg of emestrin was obtained,which marked an 11.71-fold increase compared to the original fermentation approach.The method is both simple and cost-effective,establishing a solid foundation for the efficient synthesis of emestrin and similar compounds.Additionally,it serves as an important reference for enhancing the production of other epipolythiodioxopiperazine compounds.
关键词
前体物质/谷胱甘肽/emestrin/产量提高/培养基优化/Emericella/sp/多硫代二酮哌嗪Key words
biosynthetic precursor/glutathione/emestrin/yield improvement/fermentation medium optimization/Emericella sp./epipolythiodioxopiperazine引用本文复制引用
基金项目
中国医科院医学与健康科技创新工程项目(2021-I2M-1-028)
国家自然科学基金(81302675)
国家自然科学基金(82330110)
出版年
2024
NETL
NSTL
万方数据