首页|谷胱甘肽促进Emericella sp.1454中emestrin的产量提高

谷胱甘肽促进Emericella sp.1454中emestrin的产量提高

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基于Emericella sp.1454的基因组信息,结合文献分析其次级代谢产物emestrin的生物合成前体,并通过在培养基中添加前体的方法,提高emestrin产量.本研究首次发现在培养基中添加emestrin的生物合成前体—还原型谷胱甘肽可显著提高其产量.每50 g大米培养基中加入1.5 g还原型谷胱甘肽,培养15天,可以得到30.82 mg的emestrin,相较于原始发酵培养方案提升了 11.71倍.该方法既简单经济又高效,为后续高效制备emestrin类化合物奠定了坚实的基础,以及为其他类多硫代二酮哌嗪类化合物的产量提升提供了重要的借鉴.
Enhancing production of emestrin in Emericella sp.1454 by adding the biosynthetic precursor glutathione
Based on the genomic information of Emericella sp.1454,in conjunction with literature analysis of its secondary metabolite emestrin,this study identified the biosynthetic precursors of emestrin and enhanced its production by supplementing the culture medium with these precursors.In this study,it was found for the first time that the addition of biosynthetic precursor,reduced glutathione,to the culture medium significantly increased emestrin yield.By incorporating 1.5 g of reduced glutathione into 50 g of rice culture medium and fermenting for 15 days,a yield of 30.82 mg of emestrin was obtained,which marked an 11.71-fold increase compared to the original fermentation approach.The method is both simple and cost-effective,establishing a solid foundation for the efficient synthesis of emestrin and similar compounds.Additionally,it serves as an important reference for enhancing the production of other epipolythiodioxopiperazine compounds.

biosynthetic precursorglutathioneemestrinyield improvementfermentation medium optimizationEmericella sp.epipolythiodioxopiperazine

陈渝川、肖同美、苏冰洁、闫璧滢、余利岩、司书毅、陈明华

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中国医学科学院、北京协和医学院,医药生物技术研究所,北京 100050

前体物质 谷胱甘肽 emestrin 产量提高 培养基优化 Emericella sp 多硫代二酮哌嗪

中国医科院医学与健康科技创新工程项目国家自然科学基金国家自然科学基金

2021-I2M-1-0288130267582330110

2024

10.16438/j.0513-4870.2023-0894

药学学报
中国药学会 中国医学科学院药物研究所

药学学报

CSTPCD北大核心
影响因子：1.274
ISSN：0513-4870
年,卷(期)：2024.59(4)
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